RapamycinFungusV1, Main, Exploration, bibRecord, 001026

Interplay of Aro80 and GATA activators in regulation of genes for catabolism of aromatic amino acids in Saccharomyces cerevisiae.

Identifieur interne : 001026 ( Main/Exploration ); précédent : 001025; suivant : 001027

Interplay of Aro80 and GATA activators in regulation of genes for catabolism of aromatic amino acids in Saccharomyces cerevisiae.

Auteurs : Kyusung Lee [Corée du Sud] ; Ji-Sook Hahn

Source :

Molecular microbiology [ 1365-2958 ] ; 2013.

RBID : pubmed:23651256

Descripteurs français

English descriptors

KwdEn :
- Amino Acids, Aromatic (metabolism), DNA, Fungal (metabolism), GATA Transcription Factors (genetics), GATA Transcription Factors (metabolism), Gene Expression Regulation, Fungal (MeSH), Promoter Regions, Genetic (MeSH), Protein Binding (MeSH), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (metabolism), Saccharomyces cerevisiae Proteins (genetics), Saccharomyces cerevisiae Proteins (metabolism), Trans-Activators (genetics), Trans-Activators (metabolism), Transcription Factors (genetics), Transcription Factors (metabolism).
MESH :
- chemical , genetics : GATA Transcription Factors, Saccharomyces cerevisiae Proteins, Trans-Activators, Transcription Factors.
- chemical , metabolism : Amino Acids, Aromatic, DNA, Fungal, GATA Transcription Factors, Saccharomyces cerevisiae Proteins, Trans-Activators, Transcription Factors.
- genetics : Saccharomyces cerevisiae.
- metabolism : Saccharomyces cerevisiae.
- Gene Expression Regulation, Fungal, Promoter Regions, Genetic, Protein Binding.

Abstract

Aro80, a member of the Zn(2)Cys(6) family proteins, activates expression of the ARO9 and ARO10 genes involved in catabolism of aromatic amino acids in response to aromatic amino acids that act as inducers. ARO9 and ARO10 are also under the control of nitrogen catabolite repression, but the direct roles for GATA factors, Gat1 and Gln3, in this regulation have not yet been elucidated. Here, we demonstrate that Aro80 is constitutively bound to its target promoters and activated by inducers at the level of transactivation. Although Aro80 also binds to its own promoter, ARO80 expression is induced only by rapamycin, but not by tryptophan. We show that Aro80 is absolutely required for Gat1 binding to the ARO9, ARO10 and ARO80 promoters upon rapamycin treatment. Gln3 binding to these promoters shows a partial requirement for Aro80. Rapamycin-dependent Gat1 and Gln3 binding to the Aro80 target promoters is not affected by tryptophan availability, suggesting that transactivation activity of Aro80 is not necessary for the recruitment of GATA factors. Rapamycin-dependent induction of Aro80 target genes also requires PP2A phosphatase complex, but not Sit4 phosphatase, acting downstream of TORC1.

DOI: 10.1111/mmi.12246
PubMed: 23651256

Affiliations:

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Le document en format XML

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<author><name sortKey="Lee, Kyusung" sort="Lee, Kyusung" uniqKey="Lee K" first="Kyusung" last="Lee">Kyusung Lee</name>
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<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>School of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-744</wicri:regionArea>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acids, Aromatic (metabolism)</term>
<term>DNA, Fungal (metabolism)</term>
<term>GATA Transcription Factors (genetics)</term>
<term>GATA Transcription Factors (metabolism)</term>
<term>Gene Expression Regulation, Fungal (MeSH)</term>
<term>Promoter Regions, Genetic (MeSH)</term>
<term>Protein Binding (MeSH)</term>
<term>Saccharomyces cerevisiae (genetics)</term>
<term>Saccharomyces cerevisiae (metabolism)</term>
<term>Saccharomyces cerevisiae Proteins (genetics)</term>
<term>Saccharomyces cerevisiae Proteins (metabolism)</term>
<term>Trans-Activators (genetics)</term>
<term>Trans-Activators (metabolism)</term>
<term>Transcription Factors (genetics)</term>
<term>Transcription Factors (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (métabolisme)</term>
<term>Acides aminés aromatiques (métabolisme)</term>
<term>Facteurs de transcription (génétique)</term>
<term>Facteurs de transcription (métabolisme)</term>
<term>Facteurs de transcription GATA (génétique)</term>
<term>Facteurs de transcription GATA (métabolisme)</term>
<term>Liaison aux protéines (MeSH)</term>
<term>Protéines de Saccharomyces cerevisiae (génétique)</term>
<term>Protéines de Saccharomyces cerevisiae (métabolisme)</term>
<term>Régions promotrices (génétique) (MeSH)</term>
<term>Régulation de l'expression des gènes fongiques (MeSH)</term>
<term>Saccharomyces cerevisiae (génétique)</term>
<term>Saccharomyces cerevisiae (métabolisme)</term>
<term>Transactivateurs (génétique)</term>
<term>Transactivateurs (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>GATA Transcription Factors</term>
<term>Saccharomyces cerevisiae Proteins</term>
<term>Trans-Activators</term>
<term>Transcription Factors</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Amino Acids, Aromatic</term>
<term>DNA, Fungal</term>
<term>GATA Transcription Factors</term>
<term>Saccharomyces cerevisiae Proteins</term>
<term>Trans-Activators</term>
<term>Transcription Factors</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteurs de transcription</term>
<term>Facteurs de transcription GATA</term>
<term>Protéines de Saccharomyces cerevisiae</term>
<term>Saccharomyces cerevisiae</term>
<term>Transactivateurs</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Saccharomyces cerevisiae</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN fongique</term>
<term>Acides aminés aromatiques</term>
<term>Facteurs de transcription</term>
<term>Facteurs de transcription GATA</term>
<term>Protéines de Saccharomyces cerevisiae</term>
<term>Saccharomyces cerevisiae</term>
<term>Transactivateurs</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Fungal</term>
<term>Promoter Regions, Genetic</term>
<term>Protein Binding</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Liaison aux protéines</term>
<term>Régions promotrices (génétique)</term>
<term>Régulation de l'expression des gènes fongiques</term>
</keywords>
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<front><div type="abstract" xml:lang="en">Aro80, a member of the Zn(2)Cys(6) family proteins, activates expression of the ARO9 and ARO10 genes involved in catabolism of aromatic amino acids in response to aromatic amino acids that act as inducers. ARO9 and ARO10 are also under the control of nitrogen catabolite repression, but the direct roles for GATA factors, Gat1 and Gln3, in this regulation have not yet been elucidated. Here, we demonstrate that Aro80 is constitutively bound to its target promoters and activated by inducers at the level of transactivation. Although Aro80 also binds to its own promoter, ARO80 expression is induced only by rapamycin, but not by tryptophan. We show that Aro80 is absolutely required for Gat1 binding to the ARO9, ARO10 and ARO80 promoters upon rapamycin treatment. Gln3 binding to these promoters shows a partial requirement for Aro80. Rapamycin-dependent Gat1 and Gln3 binding to the Aro80 target promoters is not affected by tryptophan availability, suggesting that transactivation activity of Aro80 is not necessary for the recruitment of GATA factors. Rapamycin-dependent induction of Aro80 target genes also requires PP2A phosphatase complex, but not Sit4 phosphatase, acting downstream of TORC1.</div>
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<Abstract><AbstractText>Aro80, a member of the Zn(2)Cys(6) family proteins, activates expression of the ARO9 and ARO10 genes involved in catabolism of aromatic amino acids in response to aromatic amino acids that act as inducers. ARO9 and ARO10 are also under the control of nitrogen catabolite repression, but the direct roles for GATA factors, Gat1 and Gln3, in this regulation have not yet been elucidated. Here, we demonstrate that Aro80 is constitutively bound to its target promoters and activated by inducers at the level of transactivation. Although Aro80 also binds to its own promoter, ARO80 expression is induced only by rapamycin, but not by tryptophan. We show that Aro80 is absolutely required for Gat1 binding to the ARO9, ARO10 and ARO80 promoters upon rapamycin treatment. Gln3 binding to these promoters shows a partial requirement for Aro80. Rapamycin-dependent Gat1 and Gln3 binding to the Aro80 target promoters is not affected by tryptophan availability, suggesting that transactivation activity of Aro80 is not necessary for the recruitment of GATA factors. Rapamycin-dependent induction of Aro80 target genes also requires PP2A phosphatase complex, but not Sit4 phosphatase, acting downstream of TORC1.</AbstractText>
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Interplay of Aro80 and GATA activators in regulation of genes for catabolism of aromatic amino acids in Saccharomyces cerevisiae.

Interplay of Aro80 and GATA activators in regulation of genes for catabolism of aromatic amino acids in Saccharomyces cerevisiae.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki